Fluorescence imaging can be used for visualizing cellular components or metabolic products. To allow the detection of those, fluorescent dyes are used to stain certain cell structures, or they are used as markers for reaction products.
Epifluorescence microscopy is commonly used in life sciences employing an inverted microscope. The excitation light irradiates the sample through the objective lens. The emitted fluorescence passes the same objective before reaching the detector. In this setup, only reflected excitatory light can reach the detector, greatly enhancing the signal-to-noise ratio. A dichroic mirror reflects the emitted fluorescence towards the detector. The excitatory light is transmitted. The inverted microscope takes up space only below the specimen which allows the integration of a scanning head from the top to perform scanning probe experiments.
Get in contact with us for consulting service.
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.