The Minimalist
The electrical specifications of the S-Probe are identical to our standard Red Star Headstage. In addition it has the feature of an optional bath sense connection which gives it the opportunity to operate in the 3 Electrode Mode (see below). Contact us to find out if your amplifier is supporting this mode.
The headstage has a thread connector to provide a safer electrical connection of the headstage and the pipette holder.
The headstage can be used either with an EPC 10 (revision ‘O’ or higher) patch clamp amplifier, an EPC 10 USB (revision ‘N’ or higher) patch clamp amplifier, an EPC 800 USB patch clamp amplifier or a PG 618 USB bipotentiostat.
RMS noise values are:
- 31 fA @ 1 kHz
- 72 fA @ 3 kHz
- 120 fA @ 5 kHz
- Dimensions (L x W x H): 49 x 17 x 14.5 mm / 1.92 x 0.65 x 0.55 inch, without connector
- Weight: 24 g
- 3 Electrode Mode option
- Flexible cable for reduced cable stress
- Thread-based connector to pipette holder for saver electrical connection
Order Number: 895137
Cyclic voltammogram in 3 Electrode Mode using an EPC 10 USB patch clamp amplifier
With an 3 Electrode Mode Headstage one can measure the potential difference between a third electrode (reference) and the ground (counter) electrode without having current flowing through the reference electrode. The reference electrode stays in equilibrium! The potential difference is added to the command potential for the working electrode.
With the 3 Electrode Mode Headstage the patch clamp amplifier / bipotentiostat has the performance of a high-end potentiostat/galvanostat with a scan range of +/-2 V (with EPC 10 USB revision ‘T’ or EPC 10 revision ‘U’). Contact us to find out if your amplifier is supporting this mode.
Measuring Configuration
Working electrode: Platinum microdisk electrode with 10 µm electroactive diameter.
Reference electrode: Ag/AgCl electrode
Counter (GND) electrode: Platinum
Redox mediator (bath solution): 0.7 mM FcMeOH dissolved in 0.117 M KCl
Cyclic Voltammogram
We have scanned the potential of the working electrode with respect to the reference electrode from 0 mV to 400 mV, then to -200 mV and back to 0 mV with a scan rate of 100 mV/s and recorded the current at the working electrode with a bandwidth of 3 kHz.
Analysis of the CV
The diffusion limiting current is determined as 1.2 nA which describes a microdisc electrode with 10 µm electroactive diameter at a concentration of 0.7 mM. The polarographic half wave potential is detected as +150 mV vs. Ag/AgCl.
